Equipment

The complete electroporation system for transfection of both eukaryotic and prokaryotic cells; includes both the CE module, and the PC module.

The Transfection Protocol Online Library by BioRad contains protocols obtained from the literature, developed by Bio-Rad scientists, or submitted by scientists. Access the library via: http://biorad-ads.com/transfection_protocols/

For researchers from the department of Cell Biology who would like to use the equipment, please contact Yizhi Jiang at igen@hms.harvard.edu and include 'Bio-Rad Electroporator' in the title to ensure that the message can be viewed by the correct personnel. The equipment is only available to use within the quad with restrictions. Contact us for more details.

4D-Nucleofector System with Core, X Unit Add-on.

There are cell type-specific Optimized Protocols or recommendations available in Lonza's knowledge database: https://knowledge.lonza.com

For researchers from the department of Cell Biology who would like to use the equipment, please contact Yizhi Jiang at igen@hms.harvard.edu and include 'Amaxa 4D Nucleofector' in the title ensure that the message can be viewed by the correct personnel. The equipment is only available to use within the quad with restrictions. Contact us for more details.

For first-time users, Suyog Chavan can provide consultation and training, please contact through igen@hms.harvard.edu, and include 'Illumina Miseq training' in the title to ensure that the message can be viewed by the correct personnel.

To obtain access to the Miseq, please be noted that:

1. The instrument is available Monday-Friday, each run is expected to start by 3 pm.
2. Our booking calendar is updated every Friday by 3:00pm. Contact Suyog and select open time slots for your run. Please include 'Illumina Miseq booking' in the title to ensure that the message can be viewed by the correct personnel.

If you have a full run: 

1. Make sure that you have created an Illumina account on https://www.illumina.com/index-d.html, and that you have the access to Sequence Hub to be able to log into our machine.
2. You must provide your own MiSeq Reagent kit. Make sure you completely thaw it out before coming to the Initiative lab.
3. Your library should be prepared and ready to be loaded into the cartridge.
4. You have the sample sheet ready to upload to the machine.
5. If anything goes wrong during the run, please let us know, and we can contact Illumina to do an instrument check. However, you are responsible for contacting Illumina to see if your kit or library was defective. We are not responsible for replacing any kits. 

If you don’t have a full run:

1. You may join someone else’s run. Check the calendar for joint run availability and contact Suyog. 
2. Coordinate with other users to decide who to provide the kit (usually the person that has the most samples).
3. Bring your own 4nM library.
4. Results will be ready by noontime the next day, or noontime Mondays if you run on a Friday.   

Our sorter operates Monday through Friday from 9 am to 6 pm. Wednesday afternoons (noon to 6 pm) and Thursdays (9 am to 6 pm) are reserved for BCMP users; Mondays, Tuesdays, Wednesday mornings (9am to noon) and Fridays are reserved for Cell Bio users.

For users from BCMP, please contact Anthony Banks at Anthony_Banks@hms.harvard.edu for booking times.

For users from Cell Biology:

1. please contact Sebástian Valerio at igen@hms.harvard.edu for consultation, training requests, and instrument reservations. Please include 'SONY sorter' in the title to ensure that the message can be viewed by the correct personnel.
2. For regular users, please provide your own chip.
3. We will update our instrument calendar every Friday by 3:00 pm. Contact Sebástian and select open time slots for your sorting. 
4. Sorting can be joined when any initiative member’s sorting session has space available. We use a 130um chip. 
5. Please clean up after sorting and notify Sebástian before you leave. 

* Maintaining a germ-free environment is very important to ensure satisfactory results for all users. So Bleach and DI washes are required after sorting.

The Neon Transfection pipette kit is not provided. For regular users, please purchase your own transfection pipette. 

Cell Line Data can be accessed by: 

https://www.thermofisher.com/us/en/home/life-science/cell-culture/trans…

For researchers from the Department of Cell Biology who would like to use the equipment, please contact Yizhi Jiang at igen@hms.harvard.edu, and include 'Neon Transfection' in the title to ensure the message can be viewed by the correct personnel.

A fully-automated, digital, inverted multi-channel fluorescence and transmitted light imaging system.

It is designed to quickly and easily evaluate your fluorescence in a variety of assays. The user has a 1-hour time limit on Microscope per day.

To use the equipment, please contact Yizhi Jiang at igen@hms.harvard.edu and include 'EVOS' in the title to ensure that the message can be viewed by the correct personnel.